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Protein Overview: GFA1

General Information:
Name(s) found: GFA1 / YKL104C [SGD]
Description(s) found:
Found 29 descriptions.
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  • Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 717 amino acids

    Gene Ontology:
    Cellular Component: cellular_component [ND]
    Biological Process: cell wall chitin biosynthetic process [IGILink to PubMed article]
    Molecular Function: glutamine-fructose-6-phosphate transaminase (isomerizing) activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST FIYKQIGKVS  60
   61 ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH PQRSDPEDQF VVVHNGIITN 120
  121 FRELKTLLIN KGYKFESDTD TECIAKLYLH LYNTNLQNGH DLDFHELTKL VLLELEGSYG 180
  181 LLCKSCHYPN EVIATRKGSP LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG 240
  241 LGPKKAREFE AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH 300
  301 TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG PYDHFMQKEI 360
  361 YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL IMIACGTSYH SCLATRAIFE 420
  421 ELSDIPVSVE LASDFLDRKC PVFRDDVCVF VSQSGETADT MLALNYCLER GALTVGIVNS 480
  481 VGSSISRVTH CGVHINAGPE IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG 540
  541 LKLIPGQIKQ VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE 600
  601 GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII ICNENDEVWA 660
  661 QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK GIDVDFPRNL AKSVTVE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) GFA1 PDC2
    View Details Gavin AC, et al. (2006) GFA1 MSN5 NUM1
    View Details Gavin AC, et al. (2002) ACC1 ADH1 ARO1 ATP2 BCP1 BLM10 BMH1 BMH2 BOI1 CAM1 CAR1 CCT4 CCT8 CDC6 CDC60 CLU1 CPA2 CRM1 CSE1 DAM1 DBP9 DHH1 DUG1 ECM29 EFB1 EST3 FAP1 FDH1 FKS1 GCN1 GCN20 GEA2 GFA1 GLT1 GUS1 HRP1 HSC82 IDH2 ILV1 INO4 KAP104 KAP123 KAP95 KTR3 LCB1 LCB2 LYS12 MDS3 MGA2 MIR1 MKT1 MLH2 MRPL3 NAB2 NAS6 NEW1 NOC2 NUD1 OLA1 PDA1 PDB1 PFK1 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PSA1 PSE1 PUP3 RAD51 RAD52 RFA1 RGR1 RKM1 RPA135 RPB2 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRP12 RVB2 SAM1 SAM2 SBP1 SCL1 SEC27 SEC7 SHM2 SLC1 SLX9 SMC3 SPA2 SRP1 SRV2 SSA4 TCB1 TEF1, TEF2 TEF4 TFP1 TIF1, TIF2 TSA1 TUB3 UBP6 ULP1 URA7 VAS1 YBR238C YFL006W YHB1 YHR020W YMR310C YNL313C YOL098C YRA1
    View Details Ho Y, et al. (2002) AAC1 AAC3 ACC1 ACH1 ADE5,7 ADH2 ADR1 AHA1 ARC1 ARC15 ARC18 ARC19 ARC35 ARC40 ARO1 ARP2 ARP3 ARP7 ATG11 ATG12 ATG17 ATP3 CAR2 CHK1 COP1 CPA2 CPR6 DIG1 DIG2 FET3 FET4 GCD11 GFA1 GND1 GSY1 GUS1 HAS1 HXT6 IDH2 IPP1 ISA2 KSS1 MDH1 MET18 MKT1 MSE1 NAP1 NGL2 NUG1 PHO84 PIM1 PMA1 PPX1 PRB1 PST2 PTC7 PUF3 PYC1 PYC2 REP1 RET1 REX2 RFC4 RGC1 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN10 RPN11 RPN3 RPN5 RPN6 RPN7 RPN8 RPO26 RPO31 RPT1 RPT3 RVB1 RVS167 SAH1 SEC18 SEC27 SEN1 SMC3 STE12 STE7 TBS1 TCB1 TEC1 TFP1 TIF1, TIF2 TSA2 TYR1 UBI4 UBR1 URA7 YDR239C YFL042C YGL081W YHR020W YHR033W YHR112C YKL161C YNL208W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run NAF1 No Comments Hazbun TR, et al. (2003)
    View Run YNL313C No Comments Hazbun TR, et al. (2003)
    View Run PGA1 No Comments Hazbun TR, et al. (2003)
    View Run DAD1 Dam1 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run HIR2 No Comments Green EM, et al (2005)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run DAD1 Dam1 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ASF1 sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant) Green EM, et al (2005)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #02 Alpha Factor Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..302] PSI-BLAST 966.9897 "Isomerase domain" of glucosamine 6-phosphate synthase (GLMS); Glucosamine 6-phosphate synthase, N-terminal domain
    2 View Details [303..548]
    [704..717]    		 PSI-BLAST 966.9897 "Isomerase domain" of glucosamine 6-phosphate synthase (GLMS); Glucosamine 6-phosphate synthase, N-terminal domain
    3 View Details [549..703] PSI-BLAST 966.9897 "Isomerase domain" of glucosamine 6-phosphate synthase (GLMS); Glucosamine 6-phosphate synthase, N-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • peptidase activity
    		•  1.34295853726897 bayes_pls_golite062009
  • catalytic activity
    		•  1.19663085909195 bayes_pls_golite062009
  • peptidase activity, acting on L-amino acid peptides
    		•  1.16226272745141 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.798789250029517 bayes_pls_golite062009
  • endopeptidase activity
    		•  0.759765292911898 bayes_pls_golite062009
  • binding
    		•  0.541510243044181 bayes_pls_golite062009
  • transferase activity
    		•  0.526806728662348 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle