Protein Overview: RPT4

General Information:
Name(s) found:	RPT4 / YOR259C [SGD]
Description(s) found: Found 33 descriptions. SHOW ALL	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for spindle pole body duplication; localized mainly to the nucleus throughout the cell cycle [SGD]
Organism:	Saccharomyces cerevisiae
Length:	437 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] proteasome regulatory particle, base subcomplex [IDA][IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	ATPase activity [IDA][TAS] endopeptidase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEEQDPLLA GLGETSGDNH TQQSHEQQPE QPQETEEHHE EEPSRVDPEQ EAHNKALNQF  60
   61 KRKLLEHRRY DDQLKQRRQN IRDLEKLYDK TENDIKALQS IGQLIGEVMK ELSEEKYIVK 120
  121 ASSGPRYIVG VRNSVDRSKL KKGVRVTLDI TTLTIMRILP RETDPLVYNM TSFEQGEITF 180
  181 DGIGGLTEQI RELREVIELP LKNPEIFQRV GIKPPKGVLL YGPPGTGKTL LAKAVAATIG 240
  241 ANFIFSPASG IVDKYIGESA RIIREMFAYA KEHEPCIIFM DEVDAIGGRR FSEGTSADRE 300
  301 IQRTLMELLT QMDGFDNLGQ TKIIMATNRP DTLDPALLRP GRLDRKVEIP LPNEAGRLEI 360
  361 FKIHTAKVKK TGEFDFEAAV KMSDGFNGAD IRNCATEAGF FAIRDDRDHI NPDDLMKAVR 420
  421 KVAEVKKLEG TIEYQKL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM29 GSG1 NAS6 OSH7 POL4 PRE5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 SWA2 TOD6 UBP6
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2002)		BLM10 CDC6 ECM29 GFA1 MLH2 NAS6 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP3 RGR1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6 YFL006W
View Details	Ho Y, et al. (2002)		ACC1 ADH4 ADR1 ARC1 ARC15 ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 AYR1 BGL2 BNI1 CBP6 CCT6 CPR6 DMA1 DMA2 DUR1,2 ENP1 ERG1 FAA1 FAA4 GAR1 GLT1 GSF2 GSP1 HEM15 HRP1 HSM3 HTA2 IDH2 IMH1 KAP104 KAP122 KGD2 KTR3 LHS1 LIA1 MAE1 MCM4 MCM7 MDN1 MET18 MKT1 MRT4 NAB2 NAS6 NDE1 NPA3 OAC1 OLA1 PAA1 PET10 PET9 PSD1 RMT2 RNR2 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RVB1 RVB2 SAP155 SAP185 SAP190 SEC53 SGD1 SHS1 SIT4 SKP1 STI1 TAP42 TFG2 TRP3 UBC12 UBI4 UTP5 VMA8 YDL063C YER138C YHM2 YHR033W YJR029W YMR196W YNL035C YNL311C
View Details	Qiu et al. (2008)		NAS6 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP1 PUP2 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAS2	RPT4	2	Unpublished Fields Lab Data
View Screen	RPT5	RPT4	2	Unpublished Fields Lab Data
View Screen	RPT4	MOB2	2	Unpublished Fields Lab Data
View Screen	RPT4	ECL1	2	Unpublished Fields Lab Data
View Screen	RPT4	SKY1	2	Unpublished Fields Lab Data
View Screen	RPT3	RPT4	2	Uetz P, et al. (2000)
View Screen	MLC1	RPT4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPT4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..94]	PSI-BLAST	608.154902	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
2	View Details	[95..177]	PSI-BLAST	608.154902	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
3	View Details	[178..351]	PSI-BLAST	608.154902	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
4	View Details	[352..437]	PSI-BLAST	608.154902	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2