Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #35 Asynchronous SPB prep |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 4 | 4 | 14.0% | 0.0236 | 45384 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 3 | 3 | 10.6% | 0.0119 | 66087 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 3 | 3 | 9.8% | 0.0124 | 61907 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 3 | 3 | 16.8% | 0.0424 | 18751 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 3 | 4 | 55.1% | 0.0619 | 16135 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 9 | 10 | 23.4% | 0.0391 | 67400 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 2 | 2 | 4.2% | 0.00616 | 83973 |
[ |
FMP27 | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 1 | 1 | 0.6% | 8.65E-4 | 303478 |
[ |
HSP42 | Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress... | 1 | 1 | 6.1% | 0.00606 | 42817 |
[ |
MET28 | Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participate... | 1 | 1 | 9.1% | 0.0122 | 21590 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 26 | 36 | 16.4% | 0.0488 | 195140 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 1 | 1 | 2.8% | 0.00588 | 44585 |
[ |
MRPL3 | Mitochondrial ribosomal protein of the large subunit | 1 | 1 | 6.2% | 0.00583 | 44000 |
[ |
NOP12 | Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit;... | 1 | 1 | 3.1% | 0.00495 | 51942 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 1 | 1 | 5.3% | 0.00549 | 45407 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 13 | 15 | 14.6% | 0.0401 | 94104 |
[ |
PAM1 | Essential protein of unknown function; exhibits variable expression during colony morphogenesis; ove... | 1 | 2 | 2.2% | 0.00548 | 92872 |
[ |
PFK2 | Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaero... | 1 | 1 | 1.0% | 0.00237 | 104618 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 1 | 9.4% | 0.00766 | 33715 |
[ |
RPS3 | Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... | 1 | 1 | 7.1% | 0.00947 | 26503 |
[ |
RRB1 | Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... | 1 | 1 | 6.3% | 0.00445 | 57261 |
[ |
Reverse_YDL140C | RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase... | 1 | 1 | 0.6% | 0.00131 | 191610 |
[ |
Reverse_YDR484W | VPS52 SGDID:S000002892, Chr IV from 1422753-1424678, Verified ORF, "Component of the GARP (Golgi-ass... | 1 | 1 | 2.3% | 0.00354 | 74333 |
[ |
Reverse_YKR031C | SPO14 SGDID:S000001739, Chr XI from 506037-500986, reverse complement, Verified ORF, "Phospholipase ... | 1 | 1 | 1.9% | 0.00135 | 195203 |
[ |
Reverse_YNL139C | RLR1 SGDID:S000005083, Chr XIV from 365719-360926, reverse complement, Verified ORF, "Subunit of the... | 1 | 1 | 0.9% | 0.00142 | 183930 |
[ |
Reverse_YPL256C | CLN2 SGDID:S000006177, Chr XVI from 66614-64977, reverse complement, Verified ORF, "G1 cyclin involv... | 1 | 1 | 2.9% | 0.00416 | 61697 |
[ |
SPA1_STAAU | (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... | 18 | 25 | 31.1% | 0.108 | 57320 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 14 | 17 | 15.4% | 0.041 | 111782 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 4 | 8 | 16.2% | 0.0719 | 29280 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 14 | 15 | 46.0% | 0.094 | 42271 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 2 | 2 | 6.5% | 0.00708 | 69768 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 2 | 2 | 6.6% | 0.00712 | 69470 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 2 | 2 | 6.9% | 0.00742 | 66602 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 2 | 2 | 6.9% | 0.00742 | 66595 |
[ |
TEF4 | Translation elongation factor EF-1 gamma | 1 | 1 | 5.1% | 0.00552 | 46520 |
[ |
UBR2 | Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation ... | 1 | 1 | 0.4% | 0.00121 | 216777 |
[ |
YBR235W | Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; Y... | 1 | 1 | 2.9% | 0.00203 | 123998 |
[ |
YCR015C | Putative protein of unknown function; YCR015C is not an essential gene | 1 | 1 | 6.9% | 0.00717 | 36278 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 8 | 8 | 79.7% | 0.264 | 7729 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 2 | 2 | 8.7% | 0.00731 | 61987 |