Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment |
Raw Data: |
Download DTASelect Filter text |
UNFILTERED Run Results:
[filter] [View our MS interpretation guidelines.] |
|||||||
Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 95 | 110 | 95.6% | 0.123 | 42271 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 65 | 69 | 95.3% | 0.111 | 29280 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 29 | 30 | 92.8% | 0.177 | 7729 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 300 | 358 | 89.8% | 0.0869 | 195140 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 12 | 15 | 85.0% | 0.0416 | 16135 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 164 | 193 | 83.5% | 0.0833 | 111782 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 19 | 20 | 81.4% | 0.0506 | 18751 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 82 | 97 | 73.5% | 0.068 | 67400 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 35 | 38 | 63.8% | 0.027 | 66087 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 16 | 16 | 55.8% | 0.0204 | 37354 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 58 | 61 | 41.2% | 0.0292 | 94104 |
[ |
RPS31 | Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... | 2 | 2 | 35.5% | 0.00536 | 17216 |
[ |
Reverse_YML026C,... | RPS18A SGDID:S000002858, Chr IV from 1359913-1359959,1360395-1360788, Verified ORF, "Protein compone... | 2 | 2 | 32.2% | 0.00554 | 17038 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 9 | 9 | 29.2% | 0.00947 | 44585 |
[ |
RPS18B, RPS18A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... | 5 | 5 | 28.8% | 0.014 | 17038 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 6 | 6 | 28.3% | 0.00823 | 33715 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 12 | 12 | 27.5% | 0.0127 | 45384 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 6 | 6 | 22.3% | 0.00358 | 74468 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 3 | 3 | 21.2% | 0.00448 | 29995 |
[ |
Reverse_YLR150W | STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... | 4 | 4 | 20.1% | 0.00595 | 29995 |
[ |
Reverse_YKR094C,... | RPL40A SGDID:S000001410, Chr IX from 68708-68715,69150-69528, Verified ORF, "Fusion protein, identic... | 1 | 1 | 19.5% | 0.00316 | 14554 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 7 | 7 | 19.0% | 0.00458 | 72105 |
[ |
RPS1A | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... | 2 | 2 | 18.0% | 0.0032 | 28743 |
[ |
RPL10 | Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... | 2 | 2 | 16.3% | 0.00369 | 25361 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 4 | 4 | 13.7% | 0.00178 | 99619 |
[ |
Reverse_YCL054W | SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... | 5 | 5 | 13.3% | 0.00242 | 96485 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 4 | 4 | 13.2% | 0.00376 | 50653 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 1 | 1 | 13.2% | 0.00384 | 10746 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 16 | 16 | 12.9% | 0.00348 | 218454 |
[ |
Reverse_YDR172W | SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF... | 2 | 2 | 12.7% | 0.00119 | 76551 |
[ |
Reverse_YGL075C | MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential memb... | 2 | 2 | 12.7% | 0.0021 | 44586 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 2 | 2 | 12.3% | 0.00172 | 52628 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 3 | 3 | 11.9% | 0.00199 | 66595 |
[ |
Reverse_YOL039W | RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a comp... | 1 | 1 | 11.3% | 0.00381 | 10746 |
[ |
Reverse_YDL229W | SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome... | 2 | 2 | 11.1% | 0.00133 | 66602 |
[ |
Reverse_YNL209W | SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribos... | 2 | 2 | 11.1% | 0.00133 | 66595 |
[ |
Reverse_YGL173C | KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... | 6 | 6 | 10.9% | 0.0016 | 175459 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 3 | 3 | 10.7% | 0.00145 | 93289 |
[ |
Reverse_YDR356W | SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... | 6 | 6 | 10.3% | 0.00259 | 111782 |
[ |
Reverse_YPL124W | SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (S... | 2 | 2 | 10.3% | 0.00321 | 29280 |
[ |
Reverse_YPL255W | BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pol... | 2 | 2 | 10.1% | 0.00211 | 45384 |
[ |
Reverse_YLR167W | RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to... | 1 | 1 | 9.9% | 0.00266 | 17216 |
[ |
Reverse_YAL047C | SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the ... | 4 | 4 | 9.6% | 0.00262 | 72105 |
[ |
Reverse_YNL126W | SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... | 2 | 2 | 9.3% | 9.62E-4 | 98227 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 4 | 4 | 9.0% | 0.00193 | 98227 |
[ |
Reverse_YLR441C,... | RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal p... | 1 | 1 | 8.6% | 0.00159 | 28743 |
[ |
Reverse_YML063W,... | RPS1B SGDID:S000004528, Chr XIII from 146482-147249, Verified ORF, "Ribosomal protein 10 (rp10) of t... | 1 | 1 | 8.6% | 0.00159 | 28812 |
[ |
Reverse_YPL131W,... | RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60... | 1 | 1 | 8.4% | 0.00137 | 33715 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 4 | 4 | 8.3% | 0.00122 | 151652 |
[ |
Reverse_YNL014W | HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... | 4 | 4 | 8.1% | 0.00156 | 115868 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 7 | 7 | 7.9% | 0.00187 | 175459 |
[ |
YTM1 | Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... | 2 | 2 | 7.8% | 0.00177 | 51359 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 4 | 4 | 7.7% | 0.00231 | 80900 |
[ |
Reverse_YOR373W | NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole bod... | 3 | 3 | 7.6% | 0.00143 | 94104 |
[ |
Reverse_YKR095W | MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... | 10 | 10 | 7.6% | 0.00217 | 218455 |
[ |
Reverse_YJR053W | BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... | 3 | 3 | 7.3% | 0.00213 | 66087 |
[ |
Reverse_YLR457C | NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... | 2 | 2 | 6.9% | 0.00255 | 37354 |
[ |
Reverse_YMR186W | HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... | 2 | 2 | 6.8% | 0.00115 | 80900 |
[ |
Reverse_YKL042W | SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindl... | 2 | 2 | 6.6% | 0.00224 | 42271 |
[ |
Reverse_YDR385W,... | EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also ... | 3 | 3 | 6.4% | 0.00145 | 93289 |
[ |
HEF3 | Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... | 3 | 3 | 6.1% | 0.00117 | 115869 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 3 | 3 | 5.8% | 0.00148 | 96825 |
[ |
Reverse_YNL188W | KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... | 1 | 1 | 4.8% | 9.39E-4 | 50653 |
[ |
Reverse_YLR075W | RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (6... | 1 | 1 | 4.5% | 0.00184 | 25361 |
[ |
Reverse_YMR129W | POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... | 2 | 2 | 4.2% | 6.09E-4 | 151652 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 1 | 1 | 4.1% | 9.32E-4 | 46914 |
[ |
Reverse_YIL149C | MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... | 5 | 5 | 4.0% | 0.00121 | 195140 |
[ |
Reverse_YJR002W | MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, wh... | 2 | 2 | 3.9% | 0.00137 | 66953 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 1 | 1 | 3.9% | 6.87E-4 | 66953 |
[ |
Reverse_YLR342W | FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... | 3 | 3 | 3.7% | 6.51E-4 | 214849 |
[ |
SPB1 | AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... | 3 | 3 | 3.2% | 0.00145 | 96485 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 3 | 3 | 3.1% | 0.0019 | 69768 |
[ |
Reverse_YNL225C | CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... | 2 | 2 | 3.1% | 0.0014 | 67400 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 1 | 1 | 2.9% | 4.93E-4 | 95153 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 2 | 2 | 2.1% | 4.34E-4 | 214849 |
[ |
Reverse_YDR212W | TCP1 SGDID:S000002620, Chr IV from 887227-888906, Verified ORF, "Alpha subunit of chaperonin-contain... | 1 | 1 | 2.1% | 7.27E-4 | 60481 |
[ |
Reverse_YHR174W | ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hyd... | 1 | 1 | 1.8% | 9.3E-4 | 46914 |
[ |
Reverse_YGR254W | ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hyd... | 1 | 1 | 1.8% | 9.3E-4 | 46816 |
[ |
Reverse_YAL005C | SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved ... | 1 | 1 | 1.7% | 6.34E-4 | 69767 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 1 | 1 | 1.6% | 7.24E-4 | 61495 |
[ |
SUP35 | Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... | 1 | 1 | 1.2% | 5.95E-4 | 76551 |
[ |
Reverse_YJL034W | KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import in... | 2 | 2 | 1.2% | 0.00119 | 74468 |
[ |
Reverse_YHR172W | SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... | 1 | 1 | 1.1% | 4.94E-4 | 96825 |
[ |
Reverse_YGL008C | PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membran... | 1 | 1 | 0.9% | 4.43E-4 | 99619 |