Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #05 Alpha Factor Prep2-TiO2 Flowthrough |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 132 | 291 | 65.5% | 0.0635 | 195140 |
[ |
RPL40B, RPL40A | Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... | 5 | 98 | 47.7% | 0.281 | 14554 |
[ |
RPS31 | Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... | 5 | 98 | 40.1% | 0.236 | 17216 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 5 | 98 | 16.0% | 0.0943 | 42826 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 23 | 42 | 32.0% | 0.0163 | 111782 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 10 | 28 | 17.3% | 0.0122 | 93289 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 12 | 23 | 25.3% | 0.0138 | 66602 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 12 | 23 | 25.3% | 0.0138 | 66595 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 11 | 22 | 24.8% | 0.0126 | 69768 |
[ |
RPL10 | Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... | 11 | 18 | 39.8% | 0.0299 | 25361 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 10 | 14 | 24.9% | 0.00911 | 61495 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 6 | 13 | 19.7% | 0.0109 | 46914 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 9 | 13 | 15.9% | 0.00519 | 99619 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 6 | 12 | 21.8% | 0.0121 | 42271 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 7 | 10 | 79.7% | 0.0531 | 7729 |
[ |
RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... | 5 | 10 | 28.6% | 0.0144 | 28812 |
[ |
RPS1A | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... | 5 | 10 | 27.5% | 0.0144 | 28743 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 6 | 9 | 10.5% | 0.00568 | 67400 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 2 | 8 | 9.5% | 0.0116 | 29280 |
[ |
HEF3 | Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... | 2 | 8 | 2.3% | 0.00281 | 115869 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 5 | 7 | 5.4% | 0.00447 | 66087 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 5 | 7 | 4.2% | 0.00137 | 218454 |
[ |
Reverse_YCL054W | SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... | 2 | 6 | 3.0% | 0.00261 | 96485 |
[ |
Reverse_YLR167W | RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to... | 2 | 5 | 10.5% | 0.012 | 17216 |
[ |
Reverse_YIL149C | MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... | 4 | 5 | 3.0% | 0.00109 | 195140 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 2 | 5 | 0.9% | 9.77E-4 | 214849 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 3 | 4 | 12.4% | 0.00335 | 46816 |
[ |
RPL13B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... | 2 | 4 | 10.6% | 0.00737 | 22525 |
[ |
Reverse_YNL014W | HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... | 4 | 4 | 4.6% | 0.0014 | 115868 |
[ |
Reverse_YJL034W | KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import in... | 2 | 4 | 3.4% | 0.00215 | 74468 |
[ |
Reverse_YKR095W | MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... | 4 | 4 | 3.0% | 7.81E-4 | 218455 |
[ |
Reverse_YMR129W | POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... | 3 | 4 | 2.2% | 0.0011 | 151652 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 3 | 3 | 15.0% | 0.00345 | 37354 |
[ |
YTM1 | Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... | 2 | 3 | 10.7% | 0.00239 | 51359 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 1 | 3 | 6.1% | 0.00748 | 16135 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 3 | 3 | 5.6% | 0.00185 | 66953 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 3 | 3 | 5.4% | 0.00156 | 80900 |
[ |
Reverse_YDR385W,... | EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also ... | 2 | 3 | 4.6% | 0.0013 | 93289 |
[ |
Reverse_YGL173C | KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... | 3 | 3 | 3.3% | 7.19E-4 | 175459 |
[ |
TCP1 | Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... | 2 | 3 | 2.9% | 0.00197 | 60481 |
[ |
Reverse_YNL225C | CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... | 2 | 2 | 8.4% | 0.00126 | 67400 |
[ |
Reverse_YMR186W | HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... | 2 | 2 | 8.1% | 0.00104 | 80900 |
[ |
Reverse_YLR075W | RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (6... | 2 | 2 | 8.1% | 0.0033 | 25361 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 2 | 2.7% | 0.00247 | 33715 |
[ |
Reverse_YAL005C | SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved ... | 1 | 2 | 1.6% | 0.00114 | 69767 |
[ |
Reverse_YDR356W | SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... | 2 | 2 | 1.6% | 7.76E-4 | 111782 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 2 | 2 | 1.5% | 5.48E-4 | 151652 |
[ |
SUP35 | Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... | 1 | 2 | 1.2% | 0.00107 | 76551 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 1 | 1 | 9.9% | 0.00228 | 18751 |
[ |
RPS18B, RPS18A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... | 1 | 1 | 7.5% | 0.00251 | 17038 |
[ |
Reverse_YJR053W | BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... | 1 | 1 | 5.7% | 6.37E-4 | 66087 |
[ |
Reverse_YLR457C | NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... | 1 | 1 | 5.6% | 0.00115 | 37354 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 1 | 1 | 3.4% | 9.52E-4 | 45384 |
[ |
Reverse_YNL209W | SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribos... | 1 | 1 | 3.3% | 5.97E-4 | 66595 |
[ |
Reverse_YDL229W | SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome... | 1 | 1 | 3.3% | 5.97E-4 | 66602 |
[ |
Reverse_YLR441C,... | RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal p... | 1 | 1 | 3.1% | 0.00143 | 28743 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 1 | 1 | 2.9% | 4.43E-4 | 95153 |
[ |
Reverse_YLR150W | STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... | 1 | 1 | 2.9% | 0.00134 | 29995 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 1 | 1 | 2.7% | 4.31E-4 | 94104 |
[ |
Reverse_YDR172W | SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF... | 1 | 1 | 2.5% | 5.34E-4 | 76551 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 1 | 1 | 2.4% | 5.89E-4 | 72105 |
[ |
Reverse_YNL126W | SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... | 1 | 1 | 2.1% | 4.33E-4 | 98227 |
[ |
Reverse_YAL047C | SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the ... | 1 | 1 | 2.1% | 5.88E-4 | 72105 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 1 | 1 | 1.6% | 8.46E-4 | 50653 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 1 | 1 | 1.4% | 4.33E-4 | 98227 |
[ |
Reverse_YLR342W | FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... | 1 | 1 | 0.8% | 1.95E-4 | 214849 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 1 | 1 | 0.8% | 2.4E-4 | 175459 |
[ |
Reverse_YHR172W | SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... | 1 | 1 | 0.7% | 4.45E-4 | 96825 |