YRC Public Data Repository - Protein Structure Prediction

Protein:	gi\|188017124, gi...
Organism:	Escherichia coli O157:H7 str. EC4113
Length:	424 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for gi|188017124, gi....

Description	E-value	Query Range	Subject Range
gi\|194432032, gi... - gi\|83570734\|ref\|ZP_00922177.1\| COG0124: Histidyl-tRNA synthetase [Shigella dysenteriae 1012], gi\|194...	377.0	[0..1]	[424..1]
SYH_SHISS - Histidine--tRNA ligase OS=Shigella sonnei (strain Ss046) GN=hisS PE=3 SV=1	376.0	[0..1]	[424..1]
gi\|24052934, gi\|... - gi\|24113843\|ref\|NP_708353.1\| histidyl-tRNA synthetase [Shigella flexneri 2a str. 301], gi\|24052934\|g... gi\|30063906, gi\|... - gi\|30063906\|ref\|NP_838077.1\| histidyl-tRNA synthetase [Shigella flexneri 2a str. 2457T], gi\|30042161... SYH_SHIFL - Histidine--tRNA ligase OS=Shigella flexneri GN=hisS PE=3 SV=3	376.0	[0..1]	[424..1]
SYH_SALPA - Histidine--tRNA ligase OS=Salmonella paratyphi A (strain ATCC 9150 / SARB42) GN=hisS PE=3 SV=1 SYH_SALPK - Histidine--tRNA ligase OS=Salmonella paratyphi A (strain AKU_12601) GN=hisS PE=3 SV=1 SYH_SALPA - Histidine--tRNA ligase OS=Salmonella paratyphi A GN=hisS PE=3 SV=1	375.0	[0..1]	[424..1]
gi\|16761437 - gi\|16761437\|ref\|NP_457054.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... gi\|213425332 - gi\|213425332\|ref\|ZP_03358082.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213649664 - gi\|213649664\|ref\|ZP_03379717.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213861387 - gi\|213861387\|ref\|ZP_03385857.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|214000624 - gi\|214000624\|ref\|ZP_03411359.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213028525 - gi\|213028525\|ref\|ZP_03342972.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... gi\|213161949 - gi\|213161949\|ref\|ZP_03347659.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serova... SYH_SALTI - Histidine--tRNA ligase OS=Salmonella typhi GN=hisS PE=3 SV=3 gi\|29136491, gi\|... - gi\|29140866\|ref\|NP_804208.1\| histidyl-tRNA synthetase [Salmonella enterica subsp. enterica serovar T... SYH_SALTI - Histidyl-tRNA synthetase OS=Salmonella typhi GN=hisS PE=3 SV=3	375.0	[0..1]	[424..1]
SYH_SHIB3 - Histidine--tRNA ligase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=hisS PE=3 SV=1	375.0	[0..1]	[424..1]
gi\|238797965, gi... - gi\|77963428\|ref\|ZP_00827238.1\| COG0124: Histidyl-tRNA synthetase [Yersinia mollaretii ATCC 43969], g...	374.0	[0..1]	[424..1]
SYH_PECAS - Histidine--tRNA ligase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) GN=hisS PE=3... SYH_ERWCT - Histidine--tRNA ligase OS=Erwinia carotovora subsp. atroseptica GN=hisS PE=3 SV=1	374.0	[0..1]	[423..1]
SYH_ECOL6 - (P60907) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) SYH_ECOLI - (P60906) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) SYH_ECO57 - (P60908) Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS)	374.0	[0..2]	[424..1]
SYH_SHIDS - Histidine--tRNA ligase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=hisS PE=3 SV=1	374.0	[0..1]	[424..1]

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Predicted Domain #1

Region A:
Residues: [1-424]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP LFKRAIGEVT  60
   61 DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN QEQRLWYIGP MFRHERPQKG 120
  121 RYRQFHQLGC EVFGLQGPDI DAELIMLTAR WWRALGISEH VTLELNSIGS LEARANYRDA 180
  181 LVAFLEQHKE KLDEDCKRRM YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL 240
  241 CKLLESAGIA YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT 300
  301 PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER LRDELPGVKL 360
  361 MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK DLRSGEQTAV AQDSVAAHLR 420
  421 TLLG

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	105.0
Match:	1kmmA
Description:	Histidyl-tRNA synthetase (HisRS), C-terminal domain; Histidyl-tRNA synthetase (HisRS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.89468709683036	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.89468709683036	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.88410036819636	bayes_pls_golite062009
ligase activity	6.92073480193262	bayes_pls_golite062009
histidine-tRNA ligase activity	5.91343220471745	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	2.99105702052936	bayes_pls_golite062009
serine-tRNA ligase activity	2.46505535069103	bayes_pls_golite062009
glycine-tRNA ligase activity	2.29229136935761	bayes_pls_golite062009
threonine-tRNA ligase activity	2.18642354927	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
alanine-tRNA ligase activity	1.82594054955281	bayes_pls_golite062009
lysine-tRNA ligase activity	1.72324031407565	bayes_pls_golite062009
asparagine-tRNA ligase activity	0.921492123574877	bayes_pls_golite062009
binding	0.916222310895772	bayes_pls_golite062009
transcription regulator activity	0.375542987753806	bayes_pls_golite062009
nucleic acid binding	0.281662799812287	bayes_pls_golite062009
DNA binding	0.0309732052586011	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.00572514744734753	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: