YRC Public Data Repository - Protein Structure Prediction

Protein:	CKI1
Organism:	Saccharomyces cerevisiae
Length:	582 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for CKI1.

Description	E-value	Query Range	Subject Range
CKI1 - Choline kinase, catalyzing the first step in phosphatidylcholine synthesis via the CDP-choline (Kenn... KICH_YEAST - Choline kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CKI1 PE=1 SV=1	0.0	[1..582]	[1..582]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[4..566]	[1..496]
gi\|220710, gi\|51... - gi\|8393107\|ref\|NP_058823.1\| choline kinase alpha [Rattus norvegicus], pir\|\|A42672 choline kinase (EC...	0.0	[110..516]	[74..432]
KICH_SCHPO - Putative choline kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC13G7.12c PE=3 ... SPAC13G7.12c - choline kinase	1.0E-99	[105..576]	[22..455]
gi\|186659525, gi... - gi\|55960796\|emb\|CAI16602.1\| ethanolamine kinase 2 [Homo sapiens], gi\|186659525\|ref\|NP_060678.2\| etha...	4.0E-99	[81..521]	[21..383]
gi\|12311795 - gi\|12311795\|emb\|CAC24490.1\| choline kinase [Pisum sativum]	4.0E-97	[117..514]	[12..338]

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Predicted Domain #1

Region A:
Residues: [1-143]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVQESRPGSV RSYSVGYQAR SRSSSQRRHS LTRQRSSQRL IRTISIESDV SNITDDDDLR  60
   61 AVNEGVAGVQ LDVSETANKG PRRASATDVT DSLGSTSSEY IEIPFVKETL DASLPSDYLK 120
  121 QDILNLIQSL KISKWYNNKK IQP

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [144-191]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VAQDMNLVKI SGAMTNAIFK VEYPKLPSLL LRIYGPNIDN IIDREYEL

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [205-218]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SLYGCFVNGR FEQF

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	12.95
Match:	1j7iA
Description:	Type IIIa 3',5"-aminoglycoside phosphotransferase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	4.77705138922602	bayes_pls_golite062009
protein kinase activity	4.68131600649845	bayes_pls_golite062009
kinase activity	4.63102094866388	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.26470104655954	bayes_pls_golite062009
protein serine/threonine kinase activity	3.92556971928915	bayes_pls_golite062009
transferase activity	3.27487631214089	bayes_pls_golite062009
binding	2.31186607166478	bayes_pls_golite062009
ATP binding	1.62950013799854	bayes_pls_golite062009
adenyl ribonucleotide binding	1.59120412984243	bayes_pls_golite062009
adenyl nucleotide binding	1.57251948653832	bayes_pls_golite062009
lipid kinase activity	1.3504796246251	bayes_pls_golite062009
ribonucleotide binding	1.32931505255952	bayes_pls_golite062009
purine ribonucleotide binding	1.32676572752438	bayes_pls_golite062009
purine nucleotide binding	1.31489723493061	bayes_pls_golite062009
nucleotide binding	1.31339663939554	bayes_pls_golite062009
protein binding	1.24858883313437	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
choline kinase activity	1.18757364210199	bayes_pls_golite062009
phosphoinositide 3-kinase activity	1.10484220525143	bayes_pls_golite062009
protein tyrosine kinase activity	1.06634272225692	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.05231366895645	bayes_pls_golite062009
1-phosphatidylinositol-3-kinase activity	1.04034573968654	bayes_pls_golite062009
inositol or phosphatidylinositol kinase activity	0.97254836668426	bayes_pls_golite062009
receptor signaling protein activity	0.957217569601377	bayes_pls_golite062009
ethanolamine kinase activity	0.68804237112665	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.380769480509274	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.22871167092767	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.185842548207239	bayes_pls_golite062009
magnesium ion binding	0.134745748018448	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.0569871200546399	bayes_pls_golite062009
MAP kinase kinase activity	0.04117702615323	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [192-204]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QILARLSLKN IGP

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [219-363]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LENSKTLTKD DIRNWKNSQR IARRMKELHV GVPLLSSERK NGSACWQKIN QWLRTIEKVD  60
   61 QWVGDPKNIE NSLLCENWSK FMDIVDRYHK WLISQEQGIE QVNKNLIFCH NDAQYGNLLF 120
  121 TAPVMNTPSL YTAPSSTSLT SQSSS

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	12.95
Match:	1j7iA
Description:	Type IIIa 3',5"-aminoglycoside phosphotransferase
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [364-519]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LFPSSSNVIV DDIINPPKQE QSQDSKLVVI DFEYAGANPA AYDLANHLSE WMYDYNNAKA  60
   61 PHQCHADRYP DKEQVLNFLY SYVSHLRGGA KEPIDEEVQR LYKSIIQWRP TVQLFWSLWA 120
  121 ILQSGKLEKK EASTAITREE IGPNGKKYII KTEPES

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #5

Region A:
Residues: [520-582]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PEEDFVENDD EPEAGVSIDT FDYMAYGRDK IAVFWGDLIG LGIITEEECK NFSSFKFLDT  60
   61 SYL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: