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Protein Overview: EG11013

General Information:
Name(s) found: topA / EG11013 [EcoGene]
Description(s) found:
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  • DNA topoisomerase I, omega subunit / Topoisomerase I; omega protein [EcoGene]
  • DNA topoisomerase I [Escherichia coli K12] [nr-271106-contam.fasta]
  • DNA topoisomerase I, omega subunit [Escherichia coli K12] [nr-271106-contam.fasta]
  • [wormpep210-phg-orf-LaDeana-ecoli-contam.fasta.nr]
  • DNA topoisomerase I, omega subunit [Escherichia coli str. K-12 substr. MG1655] [Ecoli-contam-Dam1-Mind-COMA-MBP-110.fasta]
    • Organism: Escherichia coli str. K-12 substr. MG1655
    Length: 865 amino acids

    Gene Ontology:
    Cellular Component: chromosome [IEA]
    Biological Process: DNA topological change [IEA][IGILink to PubMed article]
    DNA metabolic process [IEA]
    DNA unwinding involved in replication [IEA]
    Molecular Function: nucleotide binding [IEA]
    nucleic acid binding [IEA]
    ATP binding [IEA]
    DNA binding [IEA]
    zinc ion binding [IEA]
    protein binding [IPILink to PubMed article]
    metal ion binding [IEA]
    DNA topoisomerase activity [IEA]
    DNA topoisomerase type I activity [IEA]
    DNA topoisomerase (ATP-hydrolyzing) activity [IEA]
    isomerase activity [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK  60
   61 KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA 120
  121 WHLREVIGGD DARYSRVVFN EITKNAIRQA FNKPGELNID RVNAQQARRF MDRVVGYMVS 180
  181 PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH 240
  241 QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP GAPFITSTLQ QAASTRLGFG 300
  301 VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNQYASKEN 360
  361 SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF 420
  421 RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA 480
  481 SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY 540
  541 DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP 600
  601 TCGRKMGIRT ASTGVFLGCS GYALPPKERC KTTINLVPEN EVLNVLEGED AETNALRAKR 660
  661 RCPKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL 720
  721 KMGRFGKYMA CTNEECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGVF 780
  781 LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTMVR FSRKTKQQYV 840
  841 SSEKDGKATG WSAFYVDGKW VEGKK

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..586] PSI-BLAST 1000.0 AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).
    2 View Details [587..734] FFAS03 1.11 No description for 2gaiA was found.
    3 View Details [735..865] PSI-BLAST 19.0 C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • DNA topoisomerase activity
    		•  9.28485802237784 bayes_pls_golite062009
  • DNA topoisomerase type I activity
    		•  6.20416707013367 bayes_pls_golite062009
  • isomerase activity
    		•  5.61256457337998 bayes_pls_golite062009
  • nucleic acid binding
    		•  3.37048693883188 bayes_pls_golite062009
  • DNA binding
    		•  3.2732257752308 bayes_pls_golite062009
  • binding
    		•  3.07618319631815 bayes_pls_golite062009
  • catalytic activity
    		•  1.50449367975676 bayes_pls_golite062009
    2 No functions predicted.
    3
    Term Confidence Notes
  • DNA primase activity
    		•  3.07516234808151 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  2.45202527906689 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  1.79551532005706 bayes_pls_golite062009
  • RNA polymerase activity
    		•  1.79551532005706 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.977281907364794 bayes_pls_golite062009
  • binding
    		•  0.827218793613898 bayes_pls_golite062009
  • catalytic activity
    		•  0.434839928397784 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.33473177315365 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  0.0647934199260571 bayes_pls_golite062009


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle