Protein Overview: PXA2

General Information:
Name(s) found:	PXA2 / YKL188C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322660\|ref\|NP_012733.1\| Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related [NCBI NR] gi\|486333\|emb\|CAA82031.1\| PXA2 [Saccharomyces cerevisiae] [NCBI NR] gi\|465683\|sp\|P34230.1\|PXA2_YEAST RecName: Full=Peroxisomal long-chain fatty acid import protein 1; AltName: Full=Peroxisomal ABC transporter 2 [NCBI NR] gi\|438237\|emb\|CAA53736.1\| YKL741 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S34682 probable transport protein ALD homolog - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|395237\|emb\|CAA52250.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1931633\|gb\|AAB51597.1\| peroxisomal ABC transporter 2; Pxa2p [Saccharomyces cerevisiae] [NCBI NR] Peroxisomal long-chain fatty acid import protein 1 OS=Saccharomyces cerevisiae GN=PXA2 PE=1 SV=1 [Swiss-Prot] PXA2 `Half-size` ABC transporter required for import of long-chain fatty-acids into peroxisomes [mips] Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins [SGD] PXA2, Chr XI from 86227-88788, reverse complement [yeast_orfs2] Chr XI from 86227-88788, reverse complement [yeastorf3.fasta] Chr XI from 86226-88787, reverse complement [yeastorf4.fasta] Chr XI from 88787-86226, reverse complement [yeastorf5.fasta] (P34230) Peroxisomal long-chain fatty acid import protein 1 (Peroxisomal ABC transporter 2) [4932.FASTACCERV.txt] PXA2 SGDID:S0001671, Chr XI from 88791-86230, reverse complement, Verified ORF [yeastflysequest.fasta] Homolog of the human adrenoleukodystrophy transporter; forms a heterodimer with Pxa1p of two half ATP-binding cassette transporters in the peroxisome membrane; peroxisomal ABC transporter 2 [Saccharomyces cerevisiae]gi\|438237\|emb\|CAA53736.1\| YKL741 [Sacc [nrdb_11152004_Chlamydomonas] PXA2 SGDID:S000001671, Chr XI from 88791-86230, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins; Pxa2p [Saccharomy [nr-271106-contam.fasta] PXA2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] peroxisomal ABC transporter 2; Pxa2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Peroxisomal long-chain fatty acid import protein 1 (Peroxisomal ABC transporter 2) [nr-271106-contam.fasta] YKL741 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PXA2 SGDID:S000001671, Chr XI from 88791-86230, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Peroxisomal long-chain fatty acid import protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PXA2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PXA2 SGDID:S000001671, Chr XI from 88786-86225, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins" [yeast-030211-contam.fasta] PXA2 SGDID:S000001671, Chr XI from 88786-86225, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ABC transport complex; required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; complex also includes Pxa1p" [20141208_orf_trans.fasta] YKL188C SGDID:S000001671, Chr XI from 88786-86225, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	853 amino acids
Gene Ontology:
Cellular Component:	integral to peroxisomal membrane [IDA]
Biological Process:	fatty acid transport [IDA]
Molecular Function:	ATPase activity, coupled to transmembrane movement of substances [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MISTASAFYQ KHRVNLLRSS YIILLLATLY NSNSSSSNNK TDKKDSESTV LENKKIEEGK  60
   61 ETAVDREEDE SSKEELTIVS KHSTDSEDGA IIIDKESKTN HKGGERKGKV DFLFKLLLHD 120
  121 KKCLILFITQ AILLNIRTLL SLRVATLDGQ LVSTLVRAQY ANFTKILLGK WMILGIPASF 180
  181 INSLISYTTK LCAVTINRKV SDFLLSKYLS NHHTFYSVAS AESVSEIQDN LTKDIYTFSM 240
  241 NSSLLLNQLL KPMLDLILCS FKLLTSNTSV MGEGTLALGL IVYASNSLLK LIQPNFTRLT 300
  301 MASASLESWF RSLHSNLHSS NEEIALLRGQ KRELENVDYS FYRLVLFLNR EIKARAIYDV 360
  361 ATAFVIKYTW GAAGLVLCSI PIFFKNKPSE DTLQLKEPGN DMTADFITNR RLLVTASSSI 420
  421 GRFVELKRNI QQLRGIRLRL NKFNDLLDAN KGDDEKEPRD ERCIVEYDDS RIKFENIPLI 480
  481 TPANQVLVPE LSFDLKHGNH LLIIGPNGCG KSSLFRILGG LWPIRATPNK NHQSKLIMPR 540
  541 RTVDRDCAIF YLPQRPYMGN RSTFREQIIY PDSIEQFKER YHNDYDLGDA DLIKILQLLD 600
  601 LEDLVTENMS LLLAQRTSKN DSQQLSTEDN QSPCAIKVRD AFSIVRNWSE ELTIGVQQRL 660
  661 AMARMYYHKP KFAVLDECTS AVAPEMEQRM YENAQNFGIS LISVCHRTSL WHFHNYLLKF 720
  721 DGKGGYQFGP FNPKERLCNE EKLLELNAIL DQQVPLWERK LKDLTIAKES NIIRKSETNL 780
  781 NLFEKIEDPK TSKSNALFNA NKGQRITSPT GQETSKRLPL FSQPSSSASS NLLRNNKSLN 840
  841 KKVKTKKEEG KER

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		PXA1 PXA2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BEM1	PXA2	1	Unpublished Fields Lab Data
View Screen	YCR087W	PXA2	1	Unpublished Fields Lab Data
View Screen	MDM1	PXA2	1	Unpublished Fields Lab Data
View Screen	YPT53	PXA2	1	Unpublished Fields Lab Data
View Screen	NOG1	PXA2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..85]	deduced	N/A	No confident structure predictions are available.
2	View Details	[86..207] [226..445]	PSI-BLAST	139.0	MsbA
3	View Details	[208..225] [446..800]	PSI-BLAST	139.0	MsbA
4	View Details	[801..853]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
ATPase activity, coupled to transmembrane movement of substances	5.57844095962502	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	5.57813579764186	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	5.57813579764186	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	5.5689620139321	bayes_pls_golite062009
primary active transmembrane transporter activity	5.55992639965745	bayes_pls_golite062009
active transmembrane transporter activity	5.50005167063544	bayes_pls_golite062009
lipid transporter activity	5.43964691043504	bayes_pls_golite062009
ATPase activity, coupled	4.98276715440477	bayes_pls_golite062009
ATPase activity	4.69795387297193	bayes_pls_golite062009
nucleoside-triphosphatase activity	4.45923622153365	bayes_pls_golite062009
pyrophosphatase activity	4.39781350157199	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	4.38133275795019	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4.38085303582726	bayes_pls_golite062009
transporter activity	3.71239480053329	bayes_pls_golite062009
hydrolase activity	3.14498892233323	bayes_pls_golite062009
transmembrane transporter activity	3.13960906549884	bayes_pls_golite062009
substrate-specific transporter activity	3.1318898376276	bayes_pls_golite062009
ATP binding	3.01346604299372	bayes_pls_golite062009
adenyl ribonucleotide binding	2.96271391116269	bayes_pls_golite062009
adenyl nucleotide binding	2.91131545957759	bayes_pls_golite062009
purine ribonucleotide binding	2.70262534541085	bayes_pls_golite062009
ribonucleotide binding	2.70178191749524	bayes_pls_golite062009
purine nucleotide binding	2.6583477492092	bayes_pls_golite062009
nucleotide binding	2.62374702628143	bayes_pls_golite062009
binding	1.69327117652379	bayes_pls_golite062009
peptide-transporting ATPase activity	1.37783759788533	bayes_pls_golite062009
peptide transporter activity	1.19353064661766	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.08692822631118	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
motor activity	0.87979065815418	bayes_pls_golite062009
GTPase activity	0.83849854735032	bayes_pls_golite062009
cholesterol transporter activity	0.644421039250979	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
sterol transporter activity	0.51525091036229	bayes_pls_golite062009
drug transmembrane transporter activity	0.38711726712063	bayes_pls_golite062009
protein binding	0.378225828131122	bayes_pls_golite062009
catalytic activity	0.368034275052766	bayes_pls_golite062009
fatty acid transporter activity	0.278494397068437	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
microtubule motor activity	0.11674780318211	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.0775601177068852	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2