Protein Overview: ISU2

General Information:
Name(s) found:	ISU2 / YOR226C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324800\|ref\|NP_014869.1\| Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is [NCBI NR] gi\|62900448\|sp\|Q12056.1\|ISU2_YEAST RecName: Full=Iron sulfur cluster assembly protein 2, mitochondrial; AltName: Full=Iron sulfur cluster scaffold protein 2; Flags: Precursor [NCBI NR] pir\|\|S60953 iron-sulfur cofactor synthesis protein nifU homolog YOR226c [similarity] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420521\|emb\|CAA99445.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1050778\|emb\|CAA63189.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Iron sulfur cluster assembly protein 2, mitochondrial OS=Saccharomyces cerevisiae GN=ISU2 PE=1 SV=1 [Swiss-Prot] ISU2 Mitochondrial matrix protein, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins [mips] Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable [SGD] >Q12056 (Q12056) ORF YOR226C [4932.FASTACCERV.txt] Chr XV from 762084-761614, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 761613-762083, reverse complement [yeastorf3.fasta] Chr XV from 761614-762084, reverse complement [yeastorf4.fasta] ISU2, Chr XV from 761613-762083, reverse complement [yeast_orfs2] ISU2 SGDID:S0005752, Chr XV from 762084-761614, reverse complement, Verified ORF [yeastflysequest.fasta] Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable [Saccharomyces cer [nrdb_11152004_Chlamydomonas] (Q12056) ORF YOR226c (YOR50-16) [cerv4932.FASTAC] ISU2 SGDID:S000005752, Chr XV from 762084-761614, reverse complement, Verified ORF, "Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ISU2 SGDID:S000005752, Chr XV from 762085-761615, reverse complement, Verified ORF, "Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria [yeast-contam.fasta] Iron sulfur cluster assembly protein 2, mitochondrial precursor (Iron sulfur cluster scaffold protein 2) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable; Isu2p [Saccharomy [nr-271106-contam.fasta] ISU2 SGDID:S000005752, Chr XV from 762085-761615, reverse complement, Verified ORF, "Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Iron sulfur cluster assembly protein 2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ISU2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ISU2 SGDID:S000005752, Chr XV from 762084-761614, Genome Release 64-1-1, reverse complement, Verified ORF, "Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable" [yeast-030211-contam.fasta] ISU2 SGDID:S000005752, Chr XV from 762084-761614, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved; ISU2 has a paralog, ISU1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR7500\|c0_g1_i1\|g.5224 ORF TR7500\|c0_g1_i1\|g.5224 TR7500\|c0_g1_i1\|m.5224 type:complete len:157 (+) TR7500\|c0_g1_i1:894-1364(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YOR226C SGDID:S000005752, Chr XV from 762084-761614, Genome Release 64-1-1, reverse complement, Verified ORF, "Conserved protein of the mitochondrial matrix, required for synthesis of mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding function in mitochondria during Fe/S cluster assembly; isu1 isu2 double mutant is inviable" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2940\|g.2078 ORF asmbl_2940\|g.2078 asmbl_2940\|m.2078 type:complete len:157 (+) asmbl_2940:956-1426(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2940\|g.1691 ORF ID=asmbl_2940\|g.1691 ID=asmbl_2940\|m.1691 type:complete len:157 (+) ID=asmbl_2940:957-1427(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	156 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [IDA ]
Biological Process:	cellular iron ion homeostasis [IMP][ISS] iron-sulfur cluster assembly [IMP ][ISS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFARLANPAH FKPLTGSHIT RAAKRLYHPK VIDHYTNPRN VGSMDKSLAN VGTGIVGAPA  60
   61 CGDVIKLQIQ VNDKSGIIEN VKFKTFGCGS AIASSSYMTE LVRGMSLDEA VKIKNTEIAK 120
  121 ELSLPPVKLH CSMLAEDAIK AAIKDYKTKR NPSVLH

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ERT1	ISU2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ISU2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..156]	Pfam	78.013228	NifU-like N terminal domain No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	1.25897597318598	bayes_pls_golite062009
iron ion binding	0.905595388647019	bayes_pls_golite062009
protein binding	0.257846679116775	bayes_pls_golite062009
catalytic activity	0.256292883613773	bayes_pls_golite062009
transition metal ion binding	0.192770514366253	bayes_pls_golite062009
2 iron, 2 sulfur cluster binding	0.0439466134407704	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2