Protein Overview: PLP2

General Information:
Name(s) found:	PLP2 / YOR281C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324856\|ref\|NP_014924.1\| Essential protein with similarity to phosducins, which are G-protein regulators; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP; Plp2p [Saccharomyces cerevisiae] [NCBI NR] gi\|55583863\|sp\|Q12017.1\|PLP2_YEAST RecName: Full=Phosducin-like protein 2; AltName: Full=Viral IAP-associated factor 1 homolog [NCBI NR] gi\|45269954\|gb\|AAS56358.1\| YOR281C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S67183 hypothetical protein YOR281c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420627\|emb\|CAA99507.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1279706\|emb\|CAA61786.1\| hypothetical protein [Saccharomyces cerevisiae] [NCBI NR] gi\|10716150\|gb\|AAG21890.1\|AF110514_1 IAP-associated factor Viaf1 [Saccharomyces cerevisiae] [NCBI NR] Phosducin-like protein 2 OS=Saccharomyces cerevisiae GN=PLP2 PE=1 SV=1 [Swiss-Prot] PLP2 Phosducin homologue, likely to be involved in regulation of pheromone response [mips] Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP [SGD] >Q12017 (Q12017) Hypothetical protein (IAP-associated factor Viaf1) [4932.FASTACCERV.txt] Chr XV from 847126-846266, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 846265-847125, reverse complement [yeastorf3.fasta] Chr XV from 846266-847126, reverse complement [yeastorf4.fasta] PLP2, Chr XV from 846265-847125, reverse complement [yeast_orfs2] PLP2 SGDID:S0005807, Chr XV from 847126-846266, reverse complement, Verified ORF [yeastflysequest.fasta] Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP [Saccharomyces cerevisiae]gi\|1279706\|emb\|CAA61786.1\| hypothetical [nrdb_11152004_Chlamydomonas] (Q12017) Hypothetical protein (ORF YOR281c) (IAP-associated factor Viaf1) [cerv4932.FASTAC] PLP2 SGDID:S000005807, Chr XV from 847126-846266, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-lik [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-lik [yeast-contam.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are G-protein regulators; lethality of null mutation is functionally complemented by expression of mouse phosducin- [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are G-protein regulators; interacts with CCT (chaperonin containing TCP-1) complex; lethality of null mutation is c [yeast-200209-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] IAP-associated factor Viaf1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phosducin-like protein 2 (Viral IAP-associated factor 1 homolog) [nr-271106-contam.fasta] YOR281C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] hypothetical protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP; Plp2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is com [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are G-protein regulators; interacts with CCT (chaperonin containing TCP-1) complex; lethality of null mutation is complemented by expression of mouse phosducin-like protein MgcPhLP" [yeast-bovrabprots.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP" [yeast-contam_custom.fasta] [chemostatdb.fasta] PLP2 SGDID:S000005807, Chr XV from 847128-846268, reverse complement, Verified ORF, "Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP" [SGDyeast.20101221.fasta] Phosducin-like protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PLP2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PLP2 SGDID:S000005807, Chr XV from 847129-846269, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP" [yeast-030211-contam.fasta] PLP2 SGDID:S000005807, Chr XV from 847129-846269, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein that interacts with the CCT complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP; CCT is short for chaperonin containing TCP-1; essential gene" [20141208_orf_trans.fasta] TR7051\|c0_g1_i1\|g.4894 ORF TR7051\|c0_g1_i1\|g.4894 TR7051\|c0_g1_i1\|m.4894 type:complete len:287 (+) TR7051\|c0_g1_i1:49-909(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YOR281C SGDID:S000005807, Chr XV from 847129-846269, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential protein that interacts with the CCT (chaperonin containing TCP-1) complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2972\|g.2080 ORF asmbl_2972\|g.2080 asmbl_2972\|m.2080 type:complete len:287 (+) asmbl_2972:79-939(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2972\|g.1746 ORF ID=asmbl_2972\|g.1746 ID=asmbl_2972\|m.1746 type:complete len:287 (+) ID=asmbl_2972:79-939(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	286 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IPI]
Biological Process:	positive regulation of transcription from RNA polymerase II promoter by pheromones [IMP][IPI]
Molecular Function:	GTPase inhibitor activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQNEPMFQVQ VDESEDSEWN DILRAKGVIP ERAPSPTAKL EEALEEAIAK QHENRLEDKD  60
   61 LSDLEELEDD EDEDFLEAYK IKRLNEIRKL QERSKFGEVF HINKPEYNKE VTLASQGKKY 120
  121 EGAQTNDNGE EDDGGVYVFV HLSLQSKLQS RILSHLFQSA ACKFREIKFV EIPANRAIEN 180
  181 YPESNCPTLI VYYRGEVIKN MITLLELGGN NSKMEDFEDF MVKVGAVAEG DNRLIMNRDD 240
  241 EESREERKLH YGEKKSIRSG IRGKFNVGIG GNDDGNINDD DDGFFD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		CCT2 CCT7 PLP2 VID27

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRP42	PLP2	1	Unpublished Fields Lab Data
View Screen	CDC23	PLP2	1	Unpublished Fields Lab Data
View Screen	SPE1	PLP2	1	Unpublished Fields Lab Data
View Screen	UBC7	PLP2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PLP2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..237]	PSI-BLAST	59.27013	Phosducin
2	View Details	[238..286]	PSI-BLAST	5.045757	Protein disulfide isomerase, PDI

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
protein disulfide isomerase activity	2.22938352402713	bayes_pls_golite062009
intramolecular oxidoreductase activity, transposing S-S bonds	2.22938352402713	bayes_pls_golite062009
intramolecular oxidoreductase activity, interconverting keto- and enol-groups	2.0668189994058	bayes_pls_golite062009
antioxidant activity	1.38472228155226	bayes_pls_golite062009
transcription regulator activity	1.26775679034218	bayes_pls_golite062009
protein binding	1.18479617596618	bayes_pls_golite062009
peroxidase activity	1.15636364216535	bayes_pls_golite062009
oxidoreductase activity, acting on peroxide as acceptor	1.15636364216535	bayes_pls_golite062009
disulfide oxidoreductase activity	0.893289664404565	bayes_pls_golite062009
catalytic activity	0.672192331361968	bayes_pls_golite062009
binding	0.618139748326926	bayes_pls_golite062009
peroxiredoxin activity	0.366096607480921	bayes_pls_golite062009
glutathione peroxidase activity	0.360252986649711	bayes_pls_golite062009
transcription activator activity	0.322947914353705	bayes_pls_golite062009
protein disulfide oxidoreductase activity	0.27885589181966	bayes_pls_golite062009
thioredoxin peroxidase activity	0.163136748643487	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2