Protein Overview: RPN4

General Information:
Name(s) found:	RPN4 / YDL020C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses [SGD]
Organism:	Saccharomyces cerevisiae
Length:	531 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle [IGI][IPI ]
Biological Process:	positive regulation of DNA repair [IMP] G2/M transition of mitotic cell cycle [IMP] response to arsenic [IEP][IMP] telomere maintenance [IMP] positive regulation of protein catabolic process [IMP] positive regulation of transcription [IDA][IMP]
Molecular Function:	transcription factor activity [IDA][IMP] transcription activator activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN KNKENNTFTW  60
   61 NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF TTSEATANDR QINNVHLIPN 120
  121 EIKGASETPL QKTVNLKNIM KVSDPYVPTR NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY 180
  181 EDNYNPKMQW SLQDNSAAIN NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE 240
  241 PLLSLDVTPI SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE 300
  301 DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS TATTGKRKPA 360
  361 KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN YTKKHLSSHS STNSNSKPST 420
  421 ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC 480
  481 IKILGSEGYQ KTFSRLDALT RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		DOA4 RPN4 YTA7
View Details	Krogan NJ, et al. (2006)		DPB2 DPB3 DPB4 INM1 MBA1 MCM2 POL2 RPN4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	BOI2	Boi 2 gst control	McCusker D, et al (2007)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAS2	RPN4	2	Drees BL, et al. (2001)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	RPN4	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..305]	MSA	2.283989	View MSA. No confident structure predictions are available.
2	View Details	[306..377]	PSI-BLAST	25.045757	Designed zinc finger protein
3	View Details	[378..446]	PSI-BLAST	11.69897	Transcription factor IIIA, TFIIIA
4	View Details	[447..531]	PSI-BLAST	44.529817	ZIF268

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2