Protein Overview: MPE1

General Information:
Name(s) found:	MPE1 / YKL059C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif [SGD]
Organism:	Saccharomyces cerevisiae
Length:	441 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage and polyadenylation specificity factor complex [IPI]
Biological Process:	mRNA polyadenylation [IDA][IGI][IMP][IPI] mRNA cleavage [IDA][IGI][IMP][IPI]
Molecular Function:	RNA binding [IC]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI YNPDTEEEYD  60
   61 DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN ATRYVTGRPR VLQKRQHTAT 120
  121 TTANVSGTTE EERIASMFAT QENQWEQTQE EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY 180
  181 MCYRCGGRDH WIKNCPTNSD PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM 240
  241 ITDEGKFVVQ VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP 300
  301 VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV ETFLKKQEEL 360
  361 HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG TPVPPVPLPF GIPPFPMFPM 420
  421 PFMPPTATIT NPHQADASPK K

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 YSH1 YTH1
View Details	Krogan NJ, et al. (2006)		AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
View Details	Gavin AC, et al. (2006)		CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
View Details	Gavin AC, et al. (2002)		ACT1 AIM31 CFT1 CFT2 CKA1 CLP1 ENO2 FIP1 GLC7 HHF1, HHF2 HTA1 MPE1 PAP1 PCF11 PFK1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 RSA3 SEC13 SEC31 SSA3 SSU72 SYC1 TAF6 TIF4632 TYE7 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Qiu et al. (2008)		CFT1 CLP1 MPE1 PTA1 PTI1 REF2 RNA15 SSU72

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	MPE1	CBS2	2	Unpublished Fields Lab Data
View Screen	MPE1	HAC1	2	Unpublished Fields Lab Data
View Screen	MPE1	VPS45	2	Unpublished Fields Lab Data
View Screen	MPE1	HRP1	2	Unpublished Fields Lab Data
View Screen	MPE1	YJR141W	2	Unpublished Fields Lab Data
View Screen	MPE1	IRC16	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MPE1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..69]	MSA	2.013982	View MSA. No confident structure predictions are available.
2	View Details	[70..268]	MSA	2.517977	View MSA. No confident structure predictions are available.
3	View Details	[269..441]	PSI-BLAST	18.221849	V(D)J recombination activating protein 1 (RAG1), dimerization domain
4	View Details	[227..441]	PSI-BLAST	20.0	No description for 2cklB was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2