Table S2. Assignment of GO terms for all the members of the YDR288w and YML023c DNA repair complexes. PSP
and remote homology searches yielded data for 6/8 members of these two complexes. All the ORFs in these two complexes are
essential. An asterisk denotes an overlap between co-purifying proteins and proteins detected by two-hybrid.
| ORF |
GO Terms Assignment |
Experimental and Computational Evidence |
| YLR007w (Nse1) |
| Bio. Proc.: |
DNA repair (MS, 2H, LOC, PSP) |
| Cell. Comp.: |
Nucleus (MS, 2H, LOC, PSP) |
| Mol. Func.: |
Hydrolase (MS, 2H, LOC, PSP) |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
DNA repair |
| Localization: |
Nucleus |
| Struc. Pred.: |
Nucleotide triphosphate hydrolase; Winged helix DNA-binding domain |
|
| YLR383w (Rhc18) |
| Bio. Proc.: |
DNA repair (MS, PSP) |
| Cell. Comp.: |
Nucleus (MS, PSP) |
| Mol. Func.: |
ATP binding (MS, PSP) |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
No data |
| Localization: |
No data |
| Struc. Pred.: |
Two P-loop containing nucleotide triphosphate hydrolase domains Rad50 and Smc head domains |
|
| YOL034w (Smc5) |
| Bio. Proc.: |
DNA repair (MS, PSP) |
| Cell. Comp.: |
Nucleus (MS, PSP) |
| Mol. Func.: |
ATP binding (MS, PSP) |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
None |
| Localization: |
No signal |
| Struc. Pred.: |
Two P-loop containing nucleotide triphosphate hydrolase domains Rad50 and Smc head domains |
|
| YDL105w (Qri2) |
| Bio. Proc.: |
DNA repair (MS, 2H) |
| Cell. Comp.: |
Nucleus (MS, 2H, PSP) |
| Mol. Func.: |
Unknown |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
Protein folding* |
| Localization: |
No data |
| Struc. Pred.: |
None |
|
| YER038c (Kre29) |
| Bio. Proc.: |
DNA repair (MS, 2H) |
| Cell. Comp.: |
Nucleus (MS, 2H) |
| Mol. Func.: |
Unknown |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
DNA repair* |
| Localization: |
No data |
| Struc. Pred.: |
RPEL repeat (SMART) |
|
| YEL019c (Mms21) |
| Bio. Proc.: |
DNA repair (MS, PSP) |
| Cell. Comp.: |
Nucleus (MS, PSP) |
| Mol. Func.: |
DNA binding (MS, PSP) |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
No data |
| Localization: |
No data |
| Struc. Pred.: |
Miz 1 zinc finger-containing protein family |
|
| YML023c |
| Bio. Proc.: |
DNA repair (MS, 2H, LOC) |
| Cell. Comp.: |
Nucleus (MS, 2H, LOC) |
| Mol. Func.: |
Unknown |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
Cell growth and/or maintenance* |
| Localization: |
Nucleus |
| Struc. Pred.: |
None |
|
| YDR288w |
| Bio. Proc.: |
DNA repair (MS, 2H, LOC, PSP) |
| Cell. Comp.: |
Nucleus (MS, 2H, LOC, PSP) |
| Mol. Func.: |
DNA binding |
|
| Process predicted by co-purification: |
DNA repair |
| Process predicted by two-hybrid: |
Response to DNA damage stimulus* |
| Localization: |
Nucleus |
| Struc. Pred.: |
Adenylation domain of NAD+-dependent DNA ligase; Winged helix
DNA-binding domain |
|