FRET Media

  1. 10g Yeast Extract
  2. 30 g Bacto peptone
  3. 15 ml of 5 mg/ml adenine stock
  4. 5 ml 2.5 mg/ml uracil stock
  5. 20 g Agar
  6. 930 ml dH2O
  7. Autoclave
  8. 50 ml 40% glucose stock after autoclaving

Preparation of the Yeast Strains