FRET SD Media

  1. 1.7 g Yeast Nitrogen Base without amino acids or ammonium sulfate
  2. 5 g ammonium sulfate
  3. 950 ml dH2O
  4. Typically divided 95 ml in 100 ml bottles
  5. Autoclave
  6. 5 ml 40% glucose stock to 95 ml after autoclaving

Preparation of the Yeast Strains