Fluorescence Resonance Energy Transfer (FRET)

FRET is an elegant experimental tool to examine the spatial relationship between two proteins in vivo.

This page is the first in a series of linked web pages that describe our approach. The pages include the following sections:

• Introduction to FRET
• Microscope set up
• Preparation of the yeast
• Mounting cells
• Image acquisition
• Image analysis-Spillover factors
• Determine FRETR values

To skip to a section click on a link above.

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