Plasmids & Protocols

Requesting Plasmids and Strains

Please review our information page regarding requesting plasmids and strains.

Mass Spectrometry

Protocol for purification of TAP-tagged proteins.
(Click here for printable (PDF) version.)
Information for submitting samples to the Yates lab.
Video tutorials for using the Skyline targeted proteomics tool.

Yeast Two-Hybrid

The Assembly of the 6000 GAL4-AD clones

Primers used for amplifying yeast ORFs and their "common tails"
Vectors: pOBD2 and pOAD
Strains: PJ69-4a and PJ49-4alpha

Fluorescence Microscopy

Video describing: Preparation of Live Yeast Cells for Microscopy
Protocol for Fusing GFP to the Carboxy Terminus of Your Favorite Protein (PDF Version)
Fluorescence Resonance Energy Transfer (FRET) Tutorial
We have created a number of different plasmids that are useful for tagging proteins with CFP, YFP, or DsRed. These plasmids can be used for fusions to either the N or C terminus of your protein of interest. We also supply a plasmid to create a positive control for FRET.

C-terminal tags (Wach)

N-terminal tags (Prein)

FRET positive control

Chemostat Array

Mini-stat Informational Video