FRET - Slide Preparation (Page 5)

The pad is left out to dry for a minute or so, the time depending on the humidity. The tube with the yeast is briefly vortexed, and a 3 ul sample is dropped onto the center of the pad. A standard No. 1 1/2, 22 mm square coverslip (we use Corning) is placed on top of the pad. The coverslip is not sealed since the pad will not dry out in the time it takes to image the cells.