FRET - Image analysis - Spillover factors (Page 4)
The same process is now repeated for the FRET channel. In the image the "546" button yields the FRET channel image. Again the pixel intensity data is appended to the same text file. Also note that even though we have collected a CFP image, i.e. the 480 nm emission image, we obviously do not use it to calculate the YFP spillover. We just use the same program for all image capture to insure consistency.
