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ESSENTIAL UNCHARACTERIZED ORF LIST
ORF TAP Tagged Mass Spec. Bait YFP Tagged Localized Two Hybrid Structure
Prediction
YCR013C Yes - Lethal NA Yes - Lethal NA ND Yes
YDL193W Yes - Toxic NA Yes - Healthy Yes ND Yes
YDL196W Yes - Lethal NA Yes - Lethal NA ND Yes
YDL209C (CWC2) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YDR013W (PSF1) Yes - Healthy No expression Yes - Healthy No signal Yes Yes
YDR196C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YDR267C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YDR288W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YDR339C Yes - Lethal NA Yes - Toxic No signal No positives Yes
YDR365C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YDR367W Yes - Healthy Aberrant migration on immunoblot Yes - Healthy Yes ND Yes
YDR396W Yes - Lethal NA Yes - Lethal NA ND No
YDR413C Incorrect integration NA Yes - Lethal NA Yes Yes
YDR437W Yes - Healthy Could not purify Yes - Healthy Yes Yes Yes
YDR489W (SLD5) Cassette not amplified NA Yes - Lethal NA No positives Yes
YDR527W Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YDR531W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YFR003C Yes - Healthy Yes Yes - Healthy No signal Yes Yes
YFR042W Yes - Toxic NA Yes - Toxic Yes ND No
YGL047W Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YGL098W (USE1) Yes - Lethal NA Failed PCR NA ND Yes
YGR002C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YGR024C Yes - Healthy Could not purify Incorrect integration NA Yes Yes
YGR046W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YGR145W Yes - Lethal NA Yes - Healthy Yes ND Yes
YGR198W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YGR251W Failed PCR NA Yes - Healthy Yes ND Yes
YGR277C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YHR036W Yes - Healthy Could not purify Yes - Healthy No signal ND Yes
YHR040W Yes - Healthy No expression Yes - Healthy Yes Yes Yes
YHR083W Yes - Healthy No expression Yes - Healthy Yes Yes Yes
YHR085W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YHR122W No No Yes - Healthy Yes Yes Yes
YHR197W (RIX1) Yes - Healthy Yes Yes - Healthy No signal Yes No
YIL019W Failed Transformation NA Incorrect integration NA ND No
YIL083C Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YIL091C Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YIR010W (DSN1) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YJL010C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YJL072C (PSF2) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YJL091C (GWT1) Yes - Healthy Yes Yes - Healthy Yes ND Yes
YJL097W Yes - Healthy Aberrant migration on immunoblot Yes - Healthy Yes Yes Yes
YJR012C Yes - Healthy Yes Yes - Healthy No signal No positives No
YJR013W Yes - Healthy Yes Yes - Healthy Yes Yes No
YJR023C Incorrect integration NA Incorrect integration NA Yes No
YJR041C Yes - Lethal NA Failed Transformation NA ND Yes
YJR072C Failed PCR NA Yes - Healthy Yes Yes Yes
YJR136c Yes - Healthy Yes Yes - Healthy No signal ND No
YJR141W Yes - Healthy No expression Yes - Healthy No signal No positives No
YKL014C Incorrect integration NA Yes - Lethal NA ND Yes
YKL033w Failed Transformation NA Yes - Lethal NA ND Yes
YKL082C Yes - Lethal NA Yes - Toxic Yes No positives No
YKL088W Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YKL195W Yes - Healthy Yes Yes - Healthy Yes No positives No
YKR022C Failed PCR NA Yes - Healthy Yes Yes No
YKR038C Yes - Healthy Yes Yes - Healthy Yes ND Yes
YKR079C Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YLL034C Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YLL037W Failed Transformation NA Yes - Lethal NA No positives No
YLR008C Yes - Lethal NA Yes - Lethal NA Yes Yes
YLR022C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YLR051C Yes - Healthy Could not purify Yes - Healthy Yes No positives No
YLR132C Yes - Healthy Yes Yes - Healthy Yes No positives No
YLR145W Yes - Healthy Yes Yes - Healthy Yes No positives No
YLR243W Incorrect integration NA Incorrect integration NA ND Yes
YLR424W Yes - Healthy Yes Yes - Healthy No signal Yes Yes
YLR440C Failed Transformation NA Yes - Healthy Yes ND Yes
YML023C Yes - Healthy Yes Yes - Healthy Yes Yes No
YMR134W Yes - Lethal NA Yes - Healthy Yes ND No
YMR185W Yes - Healthy Yes Yes - Healthy No signal ND Yes
YMR211W (DML1) Yes - Lethal NA Yes - Healthy Yes ND Yes
YMR298W Yes - Toxic NA Yes - Healthy Yes ND No
YNL026W Yes - Lethal NA Yes - Healthy Yes ND Yes
YNL124W (NAF1) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YNL149C Yes - Toxic NA Yes - Healthy Yes ND Yes
YNL150W Yes - Lethal NA Yes - Lethal NA ND Yes
YNL152W Failed Transformation NA Failed Transformation NA ND Yes
YNL158W Yes - Healthy Yes Yes - Healthy Yes Yes No
YNL181W Yes - Healthy Yes Yes - Healthy Yes ND Yes
YNL182C Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YNL207W (RIO2) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YNL245C (CWC25) Yes - Healthy Yes Yes - Healthy Yes No positives No
YNL260C Yes - Healthy Yes Yes - Healthy Yes Yes No
YNL310C Cassette not amplified NA Yes - Healthy No signal Yes Yes
YNL313C Yes - Healthy Yes Yes - Healthy Yes No positives Yes
YNR046W Yes - Lethal NA Yes - Healthy Yes ND Yes
YNR054C Cassette not amplified NA Yes - Lethal NA Yes Yes
YOL022C Yes - Toxic NA Incorrect integration NA Yes Yes
YOL026C Yes - Toxic NA Yes - Lethal NA ND No
YOL146W (PSF3) Cassette not amplified NA Incorrect integration NA Yes Yes
YOR004W Yes - Healthy Yes Yes - Healthy Yes ND Yes
YOR060C Yes - Healthy Could not purify Yes - Healthy Yes ND No
YOR262W Yes - Healthy Could not purify Yes - Healthy Yes ND Yes
YOR287C Yes - Lethal NA Yes - Lethal NA Yes Yes
YPL063W (TIM50) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YPL146C Incorrect integration NA Incorrect integration NA ND Yes
YPL233W (NSL1) Yes - Healthy Yes Yes - Healthy Yes Yes Yes
YPR085C Yes - Healthy No expression Yes - Healthy No signal ND Yes
YPR143W Failed Transformation NA Failed Transformation NA ND Yes
YPR169W Yes - Healthy Yes Yes - Healthy Yes No positives Yes

Legend:
NA: Not applicable
ND: Not determined
Cassette not amplified: Initial amplification of the cassette produced no detectable PCR product.
Failed transformation: G418 resistant transformants were not obtained.
Failed PCR: Amplification to check integration did not produce a product.
Incorrect integration: G418 resistant haploids do not have correctly tagged gene.
No expression: Gene is correctly tagged but tagged protein was not detected on immunoblot of cell extract.
Could not purify: Tagged protein expressed but does not bind to either IgG resin or CaM resin
Aberrant migration on immunoblot: Tagged protein expressed but migrates with apparent molecular weight vastly different from predicted molecular weight.
No signal: Gene is correctly tagged but no fluorescent signal was detected.
No positives: No positives obtained in two-hybrid screen.

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